ASTM E2647-2008 用低剪切和连续流的滴灌流生物膜反应器量化铜绿假单胞菌(Pseudomonasaeruginosa)生物膜生长的标准试验方法

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【英文标准名称】:StandardTestMethodforQuantificationofaPseudomonasaeruginosaBiofilmGrownUsingaDripFlowBiofilmReactorwithLowShearandContinuousFlow
【原文标准名称】:用低剪切和连续流的滴灌流生物膜反应器量化铜绿假单胞菌(Pseudomonasaeruginosa)生物膜生长的标准试验方法
【标准号】:ASTME2647-2008
【标准状态】:现行
【国别】:美国
【发布日期】:2008
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:biofilm;coupon;growthreactor;Pseudomonasaeruginosa;reactor;sampling;shear
【摘要】:Vegetativebiofilmbacteriaarephenotypicallydifferentfromsuspendedcellsofthesamegenotype.Biofilmgrowthreactorsareengineeredtoproducebiofilmswithspecificcharacteristics.Alteringeithertheengineeredsystemoroperatingconditionswillmodifythosecharacteristics.Thepurposeofthistestmethodistodirectauserinhowtogrow,sampleandanalyzeaPseudomonasaeruginosabiofilmunderlowfluidshearandclosetotheair/liquidinterfaceusingthedripflowreactor.ThePseudomonasaeruginosabiofilmthatgrowshasasmoothappearanceandislooselyattached.Microscopically,thebiofilmissheet-likewithfewarchitecturaldetails.Thislaboratorybiofilmcouldrepresentthosefoundonproducesprayers,onfoodprocessingconveyorbelts,oncatheters,inlungswithcysticfibrosisandoralbiofilms,forexample.Thebiofilmgeneratedinthedripflowreactorisalsosuitableforefficacytesting.Afterthe54hgrowthphaseiscomplete,theusermayaddthetreatmentinsituorharvestthecouponsandtreatthemindividually.ResearchhasshownthatP.aeruginosabiofilmsgrowninthedripflowreactorwerelessresistanttodisinfectionthanbiofilmsgrownunderhighshearconditions.1.1ThistestmethodspecifiestheoperationalparametersrequiredtogrowarepeatablePseudomonasaeruginosabiofilmclosetotheair/liquidinterfaceinareactorwithacontinuousflowofnutrientsunderlowfluidshearconditions.Theresultingbiofilmisrepresentativeofgeneralizedsituationswherebiofilmexistsattheair/liquidinterfaceunderlowfluidshearratherthanrepresentativeofoneparticularenvironment.1.2Thistestmethodusesthedripflowbiofilmreactor.Thedripflowreactor(DFR)isaplugflowreactorwithlaminarflowresultinginlowfluidshear.Thereactorisversatileandmayalsobeusedforgrowingand/orcharacterizingdifferentspeciesofbiofilms.1.3Thistestmethoddescribeshowtosampleandanalyzebiofilmforviablecells.Biofilmpopulationdensityisrecordedaslogcolonyformingunitspersurfacearea.1.4Basicmicrobiologytrainingisrequiredtoperformthistestmethod.1.5ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:J88
【国际标准分类号】:13_060_30;91_010_20
【页数】:5P.;A4
【正文语种】:英语